TB-500 (Thymosin Beta-4 Analog)

Product Overview

TB-500 is a highly advanced synthetic analog corresponding to the active actin-binding domain of the naturally occurring peptide Thymosin Beta-4 (Tβ4). Uniquely characterized by its low molecular weight, this peptide is able to travel extensively through connective tissue planes, potentially affecting injury sites far from the initial application point. TB-500 is widely utilized in regenerative research for its unparalleled ability to sequester G-actin monomers, significantly enhancing cell motility, tissue repair, and organized angiogenesis.

Technical Specifications

• Molecular Formula: C212H350N56O78S

• Molecular Weight: 4963.44 g/mol

• CAS Number: 77591-33-4

• Sequence Profile: Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES (Engineered to include the critical actin-binding WH2 motif)

Mechanism of Action

TB-500 exerts its profound cellular repair effects through key structural and signaling pathways:

• Actin Sequestration & Cell Migration: Binds directly to G-actin monomers, modulating actin polymerization dynamics. This functionally reprograms cells, allowing endothelial cells, keratinocytes, and progenitor cells to actively migrate toward wound beds.

• Integrin-Linked Kinase (ILK) Activation: Triggers ILK and downstream Akt signaling pathways, promoting vital cell survival signaling, inhibiting apoptosis in damaged tissues, and accelerating structural repair.

• Macrophage Polarization: Actively shifts macrophages from the pro-inflammatory M1 phenotype to the pro-regenerative M2 phenotype, seamlessly accelerating the transition from inflammatory damage control to tissue rebuilding.

• Angiogenesis Promotion: Interacts with HIF-1 alpha and upregulates VEGF pathways, resulting in enhanced, stable blood vessel formation and improved nutrient delivery to compromised tissue environments.

Primary Research Applications

TB-500 is a premier compound for in vitro and animal model studies investigating:

• Accelerated Dermal Wound Healing and Keratinocyte Migration

• Cardiac Tissue Repair, Cardiomyocyte Survival, and Ischemia Recovery

• Musculoskeletal Regeneration and Connective Tissue Repair

• Hair Follicle Stem Cell Activation and Angiogenic Follicle Support

• Anti-Inflammatory Modulation and Oxidative Stress Reduction

Storage & Handling

For optimal long-term stability and structural integrity, store lyophilized powder securely at -4°F (-20°C) and protect strictly from light. Reconstituted solutions should be kept refrigerated at 36°F to 46°F (2°C to 8°C).

⚠️ Important Disclaimer for Carbon Nova Labs Customers: All information provided is for educational and reference purposes only. TB-500 is sold strictly for laboratory research use only. This compound is not approved by the FDA for human consumption, therapeutic use, or clinical application. Ensure compliance with all institutional safety protocols and consult qualified professionals before conducting research.